640 research outputs found
The 'petite negative' yeast Kluyveromyces lactis has a single gene expressing pyruvate decarboxylase activity
We cloned and sequenced the pyruvate decarboxylase
(PDC; EC 4.1.1.1) structural gene KlPDCA in the
yeast Kluyveromyces lactis and found it to be allelic
to the previously isolated rag6 mutation. The putative
amino acid sequence of the KlPdcAp appeared to be
highly homologous to those of the yeast Pdc proteins
identified so far. The disruption of KIPDCA indicated
that it is the only PDC structural gene in K. lactis, as
evidenced by the lack of PDC activity and ethanol production
in the pdcAdelta strains and by the absence of
growth on glucose in the presence of respiratory inhibitors.
It was observed that expression of the KlPDCA
gene is induced by glucose at the transcriptional level.
Transcription of the gene was reduced in the ragl,
rag2, rag5 and rag8 mutants, which are defective for
the low-affinity glucose permease, phosphoglucose isomerase, hexokinase, and a positive regulator of RAG1
expression, respectively
High temperature stability of natural maghemite: a magnetic and spectroscopic study
A combined magneto-mineralogical approach is used to diagnose maghemitization in magnetic grains of basaltic rock fragments from sand dunes in the Namibian desert in SW Africa. Data were obtained from static magnetic analysis, ferromagnetic resonance (FMR) spectroscopy, micro-Raman spectroscopy and electron microscopy. Micro-Raman spectroscopy showed that the magnetic grains in the lithic fragments form oxidative solid solution series with magnetite and maghemite as end-members. The five active Raman modes at 712, 665, 507, 380 and 344 cmâ1 indicate that maghemite in the magnetic grains has well-defined structural properties. The FMR spectral analysis provides evidence for long-range dipolar coupling, which suggests intergrowth of the magnetic phases of the oxidative solid solution series. Thermomagnetic experiments and hysteresis measurements reveal a Curie temperature of about 890 K for this maghemite. Upon heating to 970 K part of the maghemite is altered to thermodynamically more stable hematite. After selective thermal decomposition of the maghemite in a protected atmosphere, the remaining magnetic phase has a Curie temperature of 850 K, characteristic for magnetite. The unique thermal stability of this natural maghemite above its Curie temperature is explained by the well-defined mineral structure, which formed during slow oxidative alteration of magnetite under arid climate condition
ANALYSE DES NUCLĂONS ĂJECTĂS DANS UNE RĂACTION QUASI ĂLASTIQUE (p, p?) A 400 MeV
Une expérience de coïncidences (p,pγ) a été réalisée avec des protons incidents de 400 MeV sur différentes cibles 24Mg, 27Al, 28Si, 60Ni auprÚs de 1'accélérateur Saturne
Response of CsI(Tl) scintillators over a large range in energy and atomic number of ions (Part I): recombination and delta -- electrons
A simple formalism describing the light response of CsI(Tl) to heavy ions,
which quantifies the luminescence and the quenching in terms of the competition
between radiative transitions following the carrier trapping at the Tl
activator sites and the electron-hole recombination, is proposed. The effect of
the delta rays on the scintillation efficiency is for the first time
quantitatively included in a fully consistent way. The light output expression
depends on four parameters determined by a procedure of global fit to
experimental data.Comment: 28 pages, 6 figures, submitted to Nucl. Inst. Meth.
Nucleon-induced reactions at intermediate energies: New data at 96 MeV and theoretical status
Double-differential cross sections for light charged particle production (up
to A=4) were measured in 96 MeV neutron-induced reactions, at TSL laboratory
cyclotron in Uppsala (Sweden). Measurements for three targets, Fe, Pb, and U,
were performed using two independent devices, SCANDAL and MEDLEY. The data were
recorded with low energy thresholds and for a wide angular range (20-160
degrees). The normalization procedure used to extract the cross sections is
based on the np elastic scattering reaction that we measured and for which we
present experimental results. A good control of the systematic uncertainties
affecting the results is achieved. Calculations using the exciton model are
reported. Two different theoretical approches proposed to improve its
predictive power regarding the complex particle emission are tested. The
capabilities of each approach is illustrated by comparison with the 96 MeV data
that we measured, and with other experimental results available in the
literature.Comment: 21 pages, 28 figure
Evidence for Spinodal Decomposition in Nuclear Multifragmentation
Multifragmentation of a ``fused system'' was observed for central collisions
between 32 MeV/nucleon 129Xe and natSn. Most of the resulting charged products
were well identified thanks to the high performances of the INDRA 4pi array.
Experimental higher-order charge correlations for fragments show a weak but non
ambiguous enhancement of events with nearly equal-sized fragments. Supported by
dynamical calculations in which spinodal decomposition is simulated, this
observed enhancement is interpreted as a ``fossil'' signal of spinodal
instabilities in finite nuclear systems.Comment: 4 pages, 4 figures, to be published in Phys. Rev. Letter
Engineering Semantic Self-composition of Services Through Tuple-Based Coordination
Service self-composition is a well-understood research area focusing on service-based applications providing new services by automatically combining pre-existing ones. In this paper we focus on tuple-based coordination, and propose a solution leveraging logic tuples and tuple spaces to support semantic self-composition for services. A full-stack description of the solution is provided, ranging from a theoretical formalisation to a technologically valuable design and implementation
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